Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]
Por um escritor misterioso
Last updated 22 março 2025
![Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]](https://dfzljdn9uc3pi.cloudfront.net/2021/11973/1/fig-5-full.png)
Background Rheumatoid arthritis (RA) is a chronic systemic inflammatory disease with persistent synovitis. In the present study, the impact of monocyte chemoattractant protein-1 (MCP-1) was explored to determine methods for the diagnosis and treatment of RA. Methods First, fibroblast-like synoviocytes (FLSs) were obtained from a collagen-induced rat RA model. Next, MCP-1-overexpression plasmid and small interfering RNA were transfected into human and rat FLSs. Cell Counting Kit-8 (CCK-8), Transwell migration and flow cytometry assays were used to analyze cell proliferation, migration and apoptosis of FLSs following MCP-1 transfections, respectively. Furthermore, western blotting was used to analyze the expression levels of p-P38, p-PI3K, PI3K, CD31, VEGF, TNF-α and IL-β in FLSs following MCP-1 transfection. In addition, reverse transcription-quantitative PCR and ELISAs were used to analyze the expression levels of C-reactive protein (CRP), estrogen receptor, MCP-1 and pentraxin-3 in patients with clinical RA, followed by correlation analysis of clinical data. Finally, expression validation, diagnostic and protein-protein interaction (PPI) network analysis of MCP-1 were performed. Results MCP-1 promoted FLS proliferation and migration, and affected the apoptosis of FLSs. In addition, the expression levels of p-P38, p-PI3K, PI3K, CD31, VEGF, TNF-α and IL-β were also affected by MCP-1. In patients with clinical RA, the expression level of MCP-1 was increased. Moreover, CRP expression level was significantly up-regulated in RA. Clinically, MCP-1 was strongly correlated with tender joint count, swollen joint count, visual analog scale for general health and disease activity score 28 (DAS28)-MCP-1, and was moderately correlated with DAS28 and DAS28-CRP. PPI analysis showed that MCP-1 mainly interacted with other inflammatory cytokines. Conclusion In conclusion, MCP-1 may play a significant regulatory role in RA, and could be used as a measurement index of clinical RA activity.
![Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]](https://www.isct-cytotherapy.org/cms/attachment/b069a85c-e6a1-4bea-b6a7-1b3849bc2715/gr2_lrg.jpg)
Rheumatoid arthritis: From synovium biology to cell-based therapy - Cytotherapy
![Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]](https://rmdopen.bmj.com/content/rmdopen/3/2/e000527/F1.large.jpg)
Synovial tissue macrophages: friend or foe?
![Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]](https://www.tandfonline.com/cms/asset/6d33527f-fb48-4fd4-8386-45f69ddff2ad/kgmi_a_2263207_f0001_oc.jpg)
Full article: Progress of linking gut microbiota and musculoskeletal health: casualty, mechanisms, and translational values
![Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]](https://ars.els-cdn.com/content/image/1-s2.0-S0753332222002359-ga1.jpg)
The enhanced mitochondrial dysfunction by cantleyoside confines inflammatory response and promotes apoptosis of human HFLS-RA cell line via AMPK/Sirt 1/NF-κB pathway activation - ScienceDirect
![Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]](https://dfzljdn9uc3pi.cloudfront.net/2021/11973/1/fig-6-full.png)
Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]
![Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]](https://onlinelibrary.wiley.com/cms/asset/9c66dd5e-8c6b-4038-96ab-fda344d9da41/cei13098-fig-0001-m.jpg)
Driving chronicity in rheumatoid arthritis: perpetuating role of myeloid cells - Alivernini - 2018 - Clinical & Experimental Immunology - Wiley Online Library
![Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]](https://media.biocompare.com/m/37/figure/1.nihms-1546465-f0003-400x300.jpg)
Vimentin Polyclonal Antibody bs-0756R from Bioss Inc.
![Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]](https://ars.els-cdn.com/content/image/1-s2.0-S0006295219301157-gr1.jpg)
The many facets of macrophages in rheumatoid arthritis - ScienceDirect
![Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]](https://media.springernature.com/m685/springer-static/image/art%3A10.1186%2Fs13075-017-1303-3/MediaObjects/13075_2017_1303_Fig2_HTML.gif)
Fibroblast-like synoviocyte metabolism in the pathogenesis of rheumatoid arthritis, Arthritis Research & Therapy
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