2571516 - safe, artist:f0f0r3, fluttershy, pony, g4, creepy, dio brando, it was me dio, it was me fluttershy, jojo, jojo reference, jojo's bizarre adventure, kono dio da, kono flutter da, meme, simple

348246 - safe, discord, fluttershy, rarity, sparkler (g1), g1, g4, crazy diamond, diavolo, fake, jojo's bizarre adventure, jonathan joestar, josuke higashikata, recolor, stand - Derpibooru

Yes, this is in fact a Jojo reference. : r/mylittlepony

Dio's Bizarre Pony Adventure (JJBA & MLP in real life)

Is this a JoJo reference?, My Little Pony: Equestria Girls

1160563 - safe, edit, edited screencap, screencap, episode:the saddle row review, g4, my little pony: friendship is magic, comparison, dio brando, idea, jojo's bizarre adventure, knife, plaid stripes, spoon, stardust crusaders, za

Draw With Me - Dio Brando - Speed Paint + Commentary (Procreate) Jojo's Bizarre Adventure Fanart

162739 - safe, trixie, dio brando, exploitable meme, jojo's bizarre adventure, make it happen - Derpibooru

Is This a JoJo Reference?

2071211 - safe, edit, edited screencap, screencap, rainbow dash, equestria girls, equestria girls series, g4, wake up!, spoiler:choose your own ending (season 2), spoiler:eqg series (season 2), caption, cropped, dio brando, face

Is This a JoJo Reference?

Is my little pony a jojo's reference!?‽, /r/ShitPostCrusaders/, JoJo's Bizarre Adventure

683757 - safe, artist:dobado, fluttershy, angry, fangs, female, higurashi no naku koro ni, japanese, parody, pixiv, rena ryuugu, slit pupils, solo, uso da - Derpibooru