Knockout! A CRISPR/Cas Gene Targeting Lab – miniPCR bio

This lab is easy for teachers to prepare, makes gene targeting accessible to students, and packs a powerful punch! Become a genetic engineer and use CRISPR/Cas to disable a gene in bacteria. Scientists use the CRISPR/Cas system to alter the DNA in living cells. Now…
Perform cutting-edge genome editing! This lab allows advanced students to use the CRISPR/Cas system to disrupt a gene in bacteria and observe the resulting phenotypic change. 

Gene Knockout

Optional follow-up: Confirm gene knockout at the molecular level.

Knockout! PCR Genotyping Experiment

CRISPR/Cas Systems: A New Biomedical and Agricultural Diagnostic Devices for Viral Diseases

miniPCR bio (@miniPCR) / X

Resources: Workshop Report: Summer 2020 Virtual CRISPR in the Classroom

miniPCR

MLH1 CRISPR Knockout and Activation Products (m)

CRISPR Systems Santa Cruz Biotechnology

CRISPR/Cas Systems: A New Biomedical and Agricultural Diagnostic Devices for Viral Diseases

Genome-wide CRISPR-Cas9 knockout library screening identified PTPMT1 in cardiolipin synthesis is crucial to survival in hypoxia in liver cancer - ScienceDirect

Resources: Workshop Report: Summer 2020 Virtual CRISPR in the Classroom